Anti-Mouse IgG Donor Beads

Homogeneous Immuno Chemiluminescence Assay (HICA) is a homogeneous immunoassay method based on energy transfer between donor beads and acceptor beads at close proximity, resulting in luminescence.

Donor beads recognize protein 1 (Tag1 label), while acceptor beads recognize protein 2 (Tag2 label). When protein 1 binds to protein 2, the distance between the beads becomes less than 200nm. Upon excitation at 680nm, the donor beads generate singlet oxygen, which diffuses to the acceptor beads. The acceptor beads then undergo a redox reaction, emitting light at 615nm. The signal intensity is directly proportional to the strength of the protein interaction.

This product features a simple operation process, requiring no washing, with fast speed and high sensitivity. It can detect weak binding.

Specification	Fill Volume
250 μg	50 μL
5 mg	1 mL
25 mg	1 mL x 5

1. This experiment is light-sensitive; ensure all procedures are performed in the dark. It is recommended to conduct preparation, sample loading, and incubation under green light (illuminance below 100 LUX). 2. This product is compatible with multi-mode microplate readers equipped with Alpha detection modules. 3. Vortex thoroughly before use. Alternatively, briefly centrifuge (2000×g, 5–10 seconds) to ensure complete sample retrieval. 4. It is advised to use the accompanying dilution buffer provided by our company for reagent preparation and sample dilution. If additional components are required, they can be directly added to this buffer. 5. To ensure comparability of experimental data across different batches, strictly control incubation temperature and duration. 6. Avoid generating bubbles during sample loading.