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Description
Monkey IFN-γ Culture Media & Tissue Extracts OneStep ELISA KitProduct Specification Antigen IFN Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Mk Purification Protein A Stability & Storage 12 months from date of receipt reconstitution, 2 to 8C as supplied. Kit Precision Intra assay: 1. 6% Inter assay: 4. 3% Sample type Cell culture supernatant; Tissue Extracts Assay type Sandwich (quantitative) Sensitivity 3. 748 pg mL Range 15. 63 pg mL 1000 pg mL Recovery Cell culture supernatant: 106%
Product Specification
| Antigen | IFN-γ |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Mk |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: 1.6%； Inter-assay: 4.3% |
| Sample type | Cell culture supernatant; Tissue Extracts |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 3.748 pg/mL |
| Range | 15.63 pg/mL – 1000 pg/mL |
| Recovery | Cell culture supernatant: 106% Tissue Extracts: 88% |
| Assay time | 60 minutes |
| Species reactivity | Mk |
| CROSS REACTIVITY | Hu |
Background
Monkey Interferon-gamma (IFN-γ) is a critical type II interferon that serves as a master regulator of innate and adaptive immunity; it is a dimerized cytokine produced predominantly by activated T lymphocytes and natural killer (NK) cells, and as the primary macrophage-activating factor, its central biological function is to promote cellular immunity against intracellular pathogens, particularly viruses and certain bacteria. Monkey IFN-γ exerts its effects by binding to a specific receptor complex, which leads to the upregulation of major histocompatibility complex (MHC) class I and II molecules to enhance antigen presentation, while also directly inhibiting viral replication, activating macrophages to kill phagocytosed pathogens, and promoting the differentiation of naïve T cells towards a Th1 phenotype. Due to the high genetic and physiological similarity between non-human primates and humans, monkey IFN-γ is widely used in preclinical research, particularly in vaccine development, infectious disease models (such as SIV), and immunotherapy studies, where its activity serves as a key biomarker for cellular immune responses.
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