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Description
Mouse TNF-α Kit (HICA)Product Specification Stability & Storage Store at 2~8C protected from light for 12 months; the reconstituted standard can be aliquoted and stored at 20C, avoiding repeated freeze thaw cycles. Background Test Principle: This kit employs a homogeneous immuno chemiluminescence assay (HICA) for the detection of cytokine concentrations. The operation is simple and requires no washing steps. The detection system consists of two types of microspheres:
Product Specification
| Stability & Storage | Store at 2~8°C protected from light for 12 months; the reconstituted standard can be aliquoted and stored at -20°C, avoiding repeated freeze-thaw cycles. |
Background
Test Principle:
This kit employs a homogeneous immuno chemiluminescence assay (HICA) for the detection of cytokine concentrations. The operation is simple and requires no washing steps.
The detection system consists of two types of microspheres: acceptor microspheres conjugated with antibody 1 targeting the protein of interest, and donor microspheres conjugated with streptavidin, along with biotin-labeled antibody 2. During the reaction, the target protein binds with the antibodies and microspheres to form an immune complex, bringing the two microspheres into close proximity. When the distance between the donor and acceptor microspheres is less than 200 nm, singlet oxygen generated upon light excitation can transfer to the acceptor microsphere, triggering chemiluminescence. Conversely, if the target protein is absent, the distance between the microspheres is too large, and no signal is produced.
By measuring the intensity of the chemiluminescent signal, quantitative analysis of the target protein can be achieved. This method is characterized by its simplicity, rapid reaction, and high sensitivity.
Components
Name |
Specification |
Component Specification |
Detection ReagentR1 |
500T |
2 mL/vial ×1 |
2000T |
8 mL/vial ×1 |
|
10000T |
40 mL/vial ×1 |
|
Detection ReagentR2 |
500T |
2 mL/vial ×1 |
2000T |
8 mL/vial ×1 |
|
10000T |
40 mL/vial ×1 |
|
Detection ReagentR3 |
500T |
5 mL/vial ×1 |
2000T |
20 mL/vial ×1 |
|
10000T |
100 mL/vial ×1 |
|
Standard |
500T |
0.015μglyophilized powder ×1 |
2000T |
0.015μglyophilized powder ×2 |
|
10000T |
0.015μglyophilized powder ×5 |
|
StandardBuffer |
500T |
6 mL/vial ×1 |
2000T |
12 mL/vial ×1 |
|
10000T |
30 mL/vial ×1 |
Note: Recommended plates are microplates (384or96-well plates, white, shallow well)
Guidelines
Reagent R3 must be protected from light. Sample addition and incubation should be performed under green light (<100 LUX).
Recalibration is required for each test, with at least duplicate wells for each standard concentration point. Use a four-parameter (weighted 1/Y²) fitting method for calculation.
Temperature and time must be strictly controlled during incubation. Microplates should be covered with a film, and a microplate reader with ALPHA function is recommended.
The dilution matrix for calibrators should match the sample matrix. Reconstituted calibrators must be used within 2 hours.
Components from different reagent kit lots must not be mixed.
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