SKU: 83827971689

Porcine IL-13 (Interleukin 13) ELISA Kit

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Description

Porcine IL-13 (Interleukin 13) ELISA KitThe Porcine IL 13 (Interleukin 13) ELISA Kit measures IL 13 in Porcine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL 13 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection

The Porcine IL-13 (Interleukin 13) ELISA Kit measures IL-13 in Porcine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL-13 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-13 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-13 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 83827971689

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Macy Davis
Belleville, US
★★★★★ 5
Get these! You won't regret it!
These are my go-to winter shoes! They are waterproof- I love them bc they don't look like your typical "rainboots"! Stylish- I get compliments everytime I wear them! Comfortable- first time I wore them they were tight through the middle of my foot, but after two wears I had them nice and broke in, now my most comfortable shoes!
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Reviewed in the United States on February 17, 2021
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KAP
Port Orchard, US
★★★★★ 3
So nice but not a good fit on my foot
I love these boots so much, they’re exactly what I wanted. Unfortunately, they are too big in the heel and flop, while too tight in the toe. I know the toe would stretch with wear, but can’t deal with a floppy heel, they pulled my socks down off my feet. I do have narrow feet. Too bad, I’m so disappointed! They seem well made and would go with everything. Comfortable, too, if they fit right.
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Reviewed in the United States on October 31, 2018
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CAlexander
Port Orchard, US
★★★★★ 5
Extremely comfy!!!
I’m from Alaska and these boots are amazing!! I’m ordering a second pair. Cute for the office, but also 100% keep your feet warm from Snow or water. These are so Comfy
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Reviewed in the United States on April 3, 2021
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KR
Fort Morgan, US
★★★★★ 5
Best boots I have ever purchased
I do appraisal work so I was looking for something cute/professional yet durable and rugged for when I work outside in all types of weather. Found it! So comfortable, so cute, and waterproof. I wear them most days for both office work and inspection work. I range between 8.5-9 and I got a 9 and they fit great both with thin socks or thick socks. I very highly recommend.
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Reviewed in the United States on October 7, 2020
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emland11
Louisville, US
★★★★★ 5
Perfect for travel... very comfortable!
These things are amazing! I bought these a couple weeks before a trip to Italy and had planned on doing a lot of walking in them. I only got to wear them a couple times before we went on our trip, but no blisters, even during LOTS of walking in them on the trip! They wore in super quickly while still keeping their durability. My feet stay dry even in lots of rain. The photos show the wear on them after a few months, including a couple weeks of travel, and weeks of wear around town. My only complaint is that there is a tiny something (a little knot of thread with glue? not sure...) that is at the top of the opening of the boot, and it rubs the back of my ankle and makes a sore. That isn't enough to make me not wear them though. There's a photo that shows this. As you can see it's super tiny, and probably not even a flaw, it just hits my ankle *just* right so that it rubs.
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Reviewed in the United States on August 10, 2018

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