SKU: 58224867203

NF-κBp105 (Nuclear factor NF-kappa-B p105 subunit) ELISA Kit (Small Sample)

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Description

NF-κBp105 (Nuclear factor NF-kappa-B p105 subunit) ELISA Kit (Small Sample)The NF Bp105 (Nuclear factor NF kappa B p105 subunit) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human NF Bp105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human NF Bp105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After

The NF-κBp105 (Nuclear factor NF-kappa-B p105 subunit) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NF-κBp105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NF-κBp105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NF-κBp105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NF-κBp105 in the samples is then determined by comparing the OD of the samples to the standard curve.

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SKU: 58224867203

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