SKU: 50036485540

Rat ATG5 ( autophagy protein 5) ELISA Kit

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Description

Rat ATG5 ( autophagy protein 5) ELISA KitThe Rat ATG5 ( autophagy protein 5) ELISA Kit measures ATG5 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ATG5 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection

The Rat ATG5 ( autophagy protein 5) ELISA Kit measures ATG5 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ATG5 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with ATG5 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of ATG5 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 50036485540

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4.3 ★★★★★
Based on 1461 reviews
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Gerri
Battle Creek, US
★★★★★ 5
An Instant Hit and Everyone's New Favorite
Color: GREEN, Size: 14 inch (Pack of 1), Style: FROG
An instant favorite! It took quite a while for my dog to break the squeaker, too. I love that it uses a water bottle for the crinkle sound, and I'm kind of glad the squeaker finally bit the dust. That works out well for us. It's tough enough that it's not ripped yet. Our dog plays with this toy every day. No fluff to pull out was definitely a plus for me. It's a great toy for the price, and I think small and larger dogs would also enjoy it. Johan is 7 months old, weighs about 40 pounds, and always chooses a favorite toy. This one replaced a worn out toy frog that was smaller, but still treasured. I was so glad that he loves this one just as much. It's a little bit longer than a regular-sized water bottle and has dangly legs. I think you could also put plastic that crinkles inside it, and they would love that too. It has a velcro closure to keep whatever you put in there inside, and he hasn't figured out how to open it so far. Update: We just bought a second one of these because he enjoyed the first one to its conclusion. It lasted longer than most toys (about 4 months with HARD use by a giant puppy), and it is his most favorite soft toy. The original didn't rip or tear, but the velcro got worn out when he figured out how to open it. I probably could have fixed it easily, but since Johan is the goodest boy, I got him a new one.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 2, 2026
J
Verified Purchase
Joan's RV Life
Chelsea, US
★★★★★ 5
Best Toy Ever! 🐾
Color: GREEN, Size: 14 inch (Pack of 1), Style: FROG
Hey, it’s me, your dog! 🐶 This Frog toy is AMAZING! No stuffing to pull out (finally), so I can chew and squeak it all I want without making a mess. The squeaker is SO satisfying, I carry it everywhere. It’s the perfect size for playing tug-of-war and fetch with my human. I’ve had it for a while now, and it’s still holding up strong—no rips or tears yet! If you love squeaky toys and want something that lasts, this frog is paws-itively the best! 🐸
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Reviewed in the United States on December 6, 2024
A
Verified Purchase
Angelique Howe
Boise, US
★★★★★ 4
Dog toy
Color: GREEN, Size: 14 inch (Pack of 1), Style: FROG
My dogs have destroyed this toy. Squeaker gone in a day and is ripped open. It was a great little toy just not for use with heavy chewers
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Reviewed in the United States on July 5, 2021
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Verified Purchase
TRP
Omaha, US
★★★★★ 5
My dog loves this
Color: GREEN, Size: 14 inch (Pack of 1), Style: FROG
Easy to insert bottles into and easy to toss. My dog loves that it both squeaks and crunches. One of his new favorite toys.
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Reviewed in the United States on January 27, 2026
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Verified Purchase
Lisa Jones
Lowell, US
★★★★★ 3
Not durable for a large breed
Color: GREEN, Size: 14 inch (Pack of 1), Style: FROG, Color: GREEN, Size: 14 inch (Pack of 1), Style: FROG
I received this toy about 2hrs ago. My dog tore a hole in it already. Not made for Rottweiler/American Bulldog breed, apparently. 🙂 Love that there isn't stuffing though, at least she's not making a mess, lol!
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Reviewed in the United States on March 22, 2025

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