SKU: 19403428085

ADT (Androsterone) ELISA Kit

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Description

ADT (Androsterone) ELISA KitThe ADT (Androsterone) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with ADT. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to ADT. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme substrate

The ADT (Androsterone) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ADT. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to ADT. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ADT in the samples is then determined by comparing the OD of the samples to the standard curve.

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SKU: 19403428085

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